[ survey of 25-OH vitamin D serum level in young women in Neyshabur]

Background and Aims: vitamin D is a steroidal hormone which is necessary for human bones' growth and development especially in young women during their pregnancy. Its deficiency is one of the main factors in metabolic bones diseases. The aim of this study was investigate level of serum 25-OH Vitamin D in the young women in Neyshabur

Materials and Methods: this cross sectional study was performed from February 2014 to January 2015 in 841 young women in the range of 15 to 40 years old who had been referred by city physicians to ACECR [Academic Center for Education, Culture and Research]. Serum 25-OH Vitamin D level was evaluated by Chemiluminescence method

Results: vitamin D deficiency [less than 10 ng/ml] was 57.31 percent in young women. Vitamin D insufficiency [10 to 30 ng/ml] was also 30.55 percent. It was also more deficient in winter 63.27 percent, comparing to summer 50.38 percent. The lowest amount of Vitamin D was seen in the group of 15 to 20 years old.

Conclusion: the enough amount of Vitamin D should be considered in women before pregnancy by regard to deficiency of 57.31 percent. So it must be inseparable part of pre-pregnancy tests

[ survey of the turbidity interference rate in 24-hour urine protein measurement before and after cold treatment in centrifuged samples]

Introduction and Aims: proteinuria means the presence of an excess of serum proteins in the urine that is very important to achieve accurate and reliable results to the physicians. The aim of this study was to evaluate the interference of turbidity in the measurement of 24-hour urine protein before and after cold treatment in turbid centrifuged samples

Materials and Methods: this experimental study was performed in ACECR [Academic Center for Education, Culture and Research], that was conducted on a random sample of 1000 people in 2015 March to September. The measurement of 24-hour urine protein was performed by using three chloral acetic acid method [TCA] and photometer

Results: ten samples [1%] just were turbid after centrifuge. There was a significant difference [P<0.05] between the samples which were turbid after centrifuge and the samples which their turbidity have removed by cold treatment. So that, in some cases the interpretation of the results after cold treatment is quite different with before [the lowest difference was 0.05 and most difference was 0.43]

Conclusion: in order to deposit interfering factors, the urine samples that are taken to measure protein and are turbid after centrifuge should be given cold treatment and centrifuge again